Product Introduction
Routine cell culture requires serum supplementation to maintain cellular proliferation. Although serum satisfies general cell culture requirements, specific background components must be removed when optimizing nutritional formulations or labeling target molecules for research. Dialysis is a commonly used removal method: serum is circulated through hollow-fiber membranes against a physiological saline buffer to maintain osmotic pressure, thereby removing small molecules with molecular weights below 10,000 Da—including amino acids, glucose, salt ions, and unbound proteins.
Serum is commonly added to cell culture media to support cell growth and serves multiple essential functions: it provides macromolecular proteins and low-molecular-weight nutrients—including amino acids, carbohydrates, and vitamins; enhances the buffering capacity of the medium; binds or neutralizes toxic substances; reduces oxidative stress and apoptosis; and supplies growth-promoting factors that aid cellular adaptation to the culture environment.
Product Application
It can be used to study proteomics, isotope labeling, cell signal transduction, and reporter gene assays.