Item Number：C3540
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Specification & Item NumberC3540-0100 (100ml)；C3540-0500 (500ml)
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Storage ConditionsRoom temperature
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Conditions Carriage2-8℃
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Validity Period (Months)36
Product Introduction
A major step in harvesting cells for chromosome karyotyping is treatment with a hypotonic saline solution to increase cell volume. Hypotonic solutions work by creating a concentration gradient across the cytoplasmic membrane and water then rushes in by active transport.
Product Application
A hypotonic solution of potassium chloride in water for use in the preparation of blood lymphocyte chromosomes-the hypotonic treatment causes the cells to swell .
Hypotonic KCl and Sodium citrate are used most frequently . Both room temperature and 37°C are used .
Generally , the higher temperature is used to increase metaphase spreading . The time of exposure will depend on cell density and type of specimen , whether on slides or in a cell pellet . Generally , the type of hypotonic treatment is determined empirically in a particular laboratory and may need to be modified from time to time .
Hypotonic KCl and Sodium citrate are used most frequently . Both room temperature and 37°C are used .
Generally , the higher temperature is used to increase metaphase spreading . The time of exposure will depend on cell density and type of specimen , whether on slides or in a cell pellet . Generally , the type of hypotonic treatment is determined empirically in a particular laboratory and may need to be modified from time to time .
Production And Quality System
Manufactured under ISO 13485 QMS and in compliance with applicable cGMP guidelines.
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