Trypsin, an animal-derived product, is the most commonly used enzyme for harvesting cells in culture.Trypsin is a pancreatic serine protease (proteolytic enzyme) with specificity for peptide bonds involving the carboxyl group of the two basic amino acids, arginine and lysine. Purified trypsin from porcine pancreas often contains a crude mixture of lipases, nucleases, polysaccharides, and proteases.

G-banding is the most widely used banding method for chromosome analysis. The dark bands are A-T rich, late replicating, heterochromatic regions of the chromosomes while the light bands are C-G rich, early replicating, euchromatic regions. High resolution: Provides a clear ribbon for the identification of subtle anomalies.Trypsin Solution B removes proteins from the chromosomes, in order for banding to occur.

VivaCell's Trypsin is designed not only to gently dissociate cells from almost any support substrates but also as well as from each other in order to actualize cell manipulation techniques in addition to other studies that require intact cell surface proteins. As a solution, Trypsin, is available in a varied array of formulations with or without EDTA. EDTA is a chelator that binds calcium and magnesium ions which may otherwise inhibit the trypsin activity. Trypsin is often the subculturing agent of choice for cell dissociation of adherent cells, although the treatment may be cytotoxic if prolonged. Over-trypsinization is a common cause of subculture problems. In a serum-free culture experiments, the trypsin reaction may be terminated by separating the cells from the solution via centrifugation or by utilizing trypsin inhibitors such as Soybean Trypsin Inhibitor (SBTI).
Predominant Characteristics

• Animal-derived source.
• Suitable for cell culture applications.
• Long-term storage when handled properly under defined conditions.

For chromosomal G-banding preparation, prepared and aged slides are treated with the enzyme trypsin and then stained with Giemsa ( GTG banding - G bands produced with trypsin and Giemsa).  This produces a series of light and dark bands that allow positive identification of each chromosome, exhibit alternating dark and light transverse bands along the longitudinal axis of the chromosomes.